10:11 AM PT -- He did it again -- cop say Bowlen tried to use the "Do you know who my father is?" tactic with police.

Officials say John was driving over 100 mph on the 101 Freeway -- driving like a maniac and scaring other drivers. Multiple people called 911 for more than a hour trying to get cops involved. Ultimately, 5 troopers joined the search and helped apprehend him.

Once the car was stopped, officers found a puppy in the vehicle -- and animal control was called to get the animal.

Officials say Bowlen was "not cooperative."

More trouble for "the blood of the city" ... the son of Denver Broncos owner Pat Bowlen was arrested for DUI in California on Sunday, TMZ Sports has learned.

31-year-old John Bowlen was pulled over by the California Highway Patrol in the Paso Robles area around 2 PM -- and during the stop, officers determined he was under the influence of alcohol.

He was hauled to a nearby station where he was booked on 2 counts of misdemeanor DUI. He's set to be released later Monday morning.

This isn't John's first brush with the law -- the former Broncos marketing employee was arrested for domestic violence in 2015 in an incident in which he was also accused of hijacking the phone of his GF when she called 911.

During the call with the 911 operator, John claimed to be the "blood of the city" and friends with the mayor, and assured officials nothing was wrong.

He was ultimately found guilty of harassment and sentenced to 2 years probation.